Tg(ACTB-Tbx1,-GFP)1Bald
Transgene Detail

Summary

• Symbol: Tg(ACTB-Tbx1,-GFP)1Bald
• Name: transgene insertion 1, Antonio Baldini
• MGI ID: MGI:5567088
• Synonyms: COET, Conditional Over-Expression of Tbx1, FFCOET
• Transgene: Tg(ACTB-Tbx1,-GFP)1Bald Location: unknown
• Alliance: Tg(ACTB-Tbx1,-GFP)1Bald page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:146886
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTB-Tbx1,-GFP)1Bald expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tbx1 (MGI:98493)

• Mutation details: The single-copy transgene comprises a chicken beta actin promoter followed by a loxP-flanked neomycin resistance cassette (neo^r) with a triple polyadenylation signal, a full-length cDNA encoding mouse T-box 1 protein (TBX1) with a Myc epitope tag, an internal ribosomal entry site (IRES) and a green fluorescent protein coding sequence. Upon cre recombinase-mediated recombination, neo^r is excised and the Tbx1 cDNA is expressed under control of the beta-actin promoter. (J:146886)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:146886 Vitelli F, et al., Gain of function of Tbx1 affects pharyngeal and heart development in the mouse. Genesis. 2009 Mar;47(3):188-95
• All: 3 reference(s)