Tg(Ins2-HBEGF)5Rin
Transgene Detail

Summary

• Symbol: Tg(Ins2-HBEGF)5Rin
• Name: transgene insertion 5, The Tokyo Metropolitan Institute of Medical Science
• MGI ID: MGI:5565217
• Synonyms: ins2(BAC)-TRECK-Tg, Tg(InsBAC-TRECK/DTR)5Rin
• Transgene: Tg(Ins2-HBEGF)5Rin Location: unknown
• Alliance: Tg(Ins2-HBEGF)5Rin page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-HBEGF)5Rin expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Tg(Ins2-HBEGF)5Rin expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins2 (MGI:96573)

• Mutation details: The RP23-92L23 bacterial artificial chromosome (BAC) containing mouse Ins2 was modified by first replacing the Ins2 coding region by galK. Then the galK insert was replaced by the human diphtheria toxin receptor (HBEGF) cDNA. This results in the human diphtheria toxin receptor under the control of the mouse Ins2 promoter. Lines 5, 14, 17, and 33 were generated. Transgene expression is seen in pancreatic beta cells and administration of diphtheria toxin results in HBEGF expressing cells. (J:204420)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:204420 Matsuoka K, et al., Generation of mouse models for type 1 diabetes by selective depletion of pancreatic beta cells using toxin receptor-mediated cell knockout. Biochem Biophys Res Commun. 2013 Jul 5;436(3):400-5
• All: 1 reference(s)