Tg(Prnp*)CDah
Transgene Detail

Summary

• Symbol: Tg(Prnp*)CDah
• Name: transgene insertion C, David A Harris
• MGI ID: MGI:5563233
• Synonyms: Tg(PG14-C)
• Transgene: Tg(Prnp*)CDah Location: unknown
• Alliance: Tg(Prnp*)CDah page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp*)CDah expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: The transgene consists of 12 kb of the mouse Prnp promoter region, which includes intron 1, and a mutant Prnp cDNA containing 9 octapeptide insertions rich in glycine and proline residues. This mutation is found in human prion diseases. The transgene also includes an epitope tag for monoclonal antibody 3F4. Hemizygous mice of line C carry four copies of the transgenic construct, as determined by quantitative Southern blot analysis. Western blot analysis of brain homogenates using the 3F4 antibody demonstrates expression of the mutant prion protein at 0.15-fold the level of endogenous hamster PrP. (J:51891)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:51891 Chiesa R, et al., Neurological illness in transgenic mice expressing a prion protein with an insertional mutation. Neuron. 1998 Dec;21(6):1339-51
• All: 2 reference(s)