Tg(Sp7/mCherry)2Pmay
Transgene Detail
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Symbol: |
Tg(Sp7/mCherry)2Pmay |
Name: |
transgene insertion 2, Peter Maye |
MGI ID: |
MGI:5556386 |
Synonyms: |
Osterix-Cherry, Osterix-mCherry |
Transgene: |
Tg(Sp7/mCherry)2Pmay Location: unknown
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Alliance: |
Tg(Sp7/mCherry)2Pmay page
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Transgene Type: |
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Transgenic (Reporter) |
Mutation: |
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Insertion
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Mutation details: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a 39 kb BAC (#RP24-362M3) containing the entire mouse Sp7 gene. This BAC was modified to fuse the first 17 amino acids of Sp7 to the N-terminal of the red fluorescent protein (Cherry) sequence upstream of the second translation start site. This transgene contains two translational start sites; the first is located in exon 1b and results in a long protein isoform containing 428 amino acids. The second start site is in-frame with upstream start site located at the beginning of exon 2 and results in a short protein isoform containing 410 amino acids. The Cherry reporter can be used to detect both isoforms. Strong reporter expression is observed in mature osteoblasts and osteocytes; weak expression is observed in bone marrow.
(J:195100)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:195100 Strecker S, et al., Generation and characterization of Osterix-Cherry reporter mice. Genesis. 2013 Apr;51(4):246-58 |
All: |
11 reference(s) |
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