Tg(EEF1A1-Ahr)1Pjvt
Transgene Detail

Summary

• Symbol: Tg(EEF1A1-Ahr)1Pjvt
• Name: transgene insertion 1, Raimo Pohjanvirta
• MGI ID: MGI:5475502
• Synonyms: rWT, Tg(EF1alfa-Ahr), Tg WT-rat AH receptor
• Transgene: Tg(EEF1A1-Ahr)1Pjvt Location: unknown
• Alliance: Tg(EEF1A1-Ahr)1Pjvt page

Transgene origin

• Strain of Origin: C57BL/6JOlaHsd

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(EEF1A1-Ahr)1Pjvt expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Ahr (25690)

  Ahr	(MGI:105043)

• Mutation details: Mice bearing this transgene express the wild-type (Long-Evans) rat aryl hydrocarbon receptor. The transgene contains the promoter of the human eukaryotic translation elongation factor-1alpha1 (EEF1A1, also called "EF1alpha") followed by a 200 bp intron, a ~90 bp fusion of most of the mouse Ahr leader sequence with the 30-bp UTR of the rat Ahr mRNA, a cDNA encoding the wild-type rat AHR, the SV40 late polyadenylation signal, and nearly 800 bp of rat Ahr 3' UTR; this entire construct is flanked 5' by a 600 bp and 3' by a 250 bp chicken beta-globin core insulator. It was estimated by real-time quantitative polymerase chain reaction (qPCR) analysis that 5 copies of the transgene are present per haploid genome. Transgene-derived mRNA is present in every one of multiple tissues analyzed by reverse transcription-PCR (RT-PCR) and has been shown to be inducible by orally administered 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the liver, where the rat protein also has been detected by western blot analysis. (J:147952)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:147952 Pohjanvirta R, Transgenic mouse lines expressing rat AH receptor variants--a new animal model for research on AH receptor function and dioxin toxicity mechanisms. Toxicol Appl Pharmacol. 2009 Apr 15;236(2):166-82
• All: 1 reference(s)