Tg(CD4-TKdelta)2Kzmn
Transgene Detail

Summary

• Symbol: Tg(CD4-TKdelta)2Kzmn
• Name: transgene insertion 2, David Klatzmann
• MGI ID: MGI:5318568
• Synonyms: EPCD4deltaTKL2, EpCD4deltaTK line 2
• Transgene: Tg(CD4-TKdelta)2Kzmn Location: unknown
• Alliance: Tg(CD4-TKdelta)2Kzmn page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Mice with this transgene express a truncated human Herpes simplex 1 (HSV1) thymidine kinase gene (TK) under control of a 2.1 kb fragment of the EpCD4 plasmid containing three tandem copies of the mouse Cd4 enhancer followed by the human CD4 promoter, without the silencer element, which drives expression in both CD4⁺ and CD8⁺ T cells. The 1-kb TK sequence begins just upstream of the second (of four) ATG translation start codons; the endogenous TK polyadenylation signal (poly[A]) has been replaced by the 0.8-kb SV40 poly[A]. Elimination of the TK upstream start sites removes a putative testis-specific enhancer and abrogates expression of TK in the testes. (J:123437, J:128760, J:183380)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

In this transgenic line, administration of gancyclovir, a nucleoside analog that is converted in dividing cells expressing HSV1-TK to a cytotoxic triphosphate form, results in ablation of >98% of dividing TK-expressing T cells. (J:183380)

References

• Original: J:183380 Bellier B, et al., Turning immunological memory into amnesia by depletion of dividing T cells. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):15017-22
• All: 4 reference(s)