Tg(Lck-CFLAR)1Zrng
Transgene Detail

Summary

• Symbol: Tg(Lck-CFLAR)1Zrng
• Name: transgene insertion 1, Martin Zoernig
• MGI ID: MGI:5317724
• Synonyms: lck-FLIP-s, Tg(Lck-cFLIP)
• Transgene: Tg(Lck-CFLAR)1Zrng Location: unknown
• Alliance: Tg(Lck-CFLAR)1Zrng page

Transgene origin

• Strain of Origin: CBA x C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Lck-CFLAR)1Zrng expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CFLAR (8837)

  Cflar

  (MGI:1336166)

• Mutation details: This transgene contains a cDNA encoding the short form of human CASP8 and FADD-like apoptosis regulator (CFLAR; also called FLIP) with an N-terminal FLAG tag inserted into the p1017 expression vector, which contains a 3.2 kb fragment of the mouse lymphocyte protein tyrosine kinase (Lck) gene that includes the thymocyte-specific proximal promoter through nucleotide +37 (relative to the transcription initiation site) upstream of the nearly full-length, 5' truncated human growth hormone (GH1) gene; the insertion site is in the truncated first exon of GH1, which encodes the 5' UTR. Western blot analysis demonstrates high levels of expression in thymocytes and splenic T cells of transgenic mice. (J:97825)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:97825 Oehme I, et al., Transgenic overexpression of the Caspase-8 inhibitor FLIP(short) leads to impaired T cell proliferation and an increased memory T cell pool after staphylococcal enterotoxin B injection. Eur J Immunol. 2005 Apr;35(4):1240-9
• All: 1 reference(s)