Tg(Pcp2-Prnp)E330Zbz
Transgene Detail

Summary

• Symbol: Tg(Pcp2-Prnp)E330Zbz
• Name: transgene insertion E330, Zurich
• MGI ID: MGI:5014494
• Synonyms: Tg(L7-Prnp)E330, Tg(PrPdelta121)E330
• Transgene: Tg(Pcp2-Prnp)E330Zbz Location: unknown
• Alliance: Tg(Pcp2-Prnp)E330Zbz page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pcp2-Prnp)E330Zbz expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: Mice bearing this transgene express a truncated mouse prion protein (PRNP) lacking amino acids 32 through 121 under control of the regulatory elements of the Purkinje cell protein 2 (L7) gene. A cDNA encoding the modified PRNP and extending to the EcoR1 site in the 3' UTR was inserted into a cloned mouse genomic DNA segment containing the entire Pcp2 coding region, 4 kb of 5' flanking sequence and 2 kb of 3' flanking sequence. Insertion occurred at the BamH1 site in the fourth (third coding) exon, so the initial translation product has 80 amino acids of PCP2/L7 joined to its amino terminus; these presumably are removed with the PRNP N-terminal leader sequence. (J:47034, J:82809, J:83952, J:173517)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:83809 Gilbert R, et al., Prolonged dystrophin expression and functional correction of mdx mouse muscle following gene transfer with a helper-dependent (gutted) adenovirus-encoding murine dystrophin. Hum Mol Genet. 2003 Jun 1;12(11):1287-99
• All: 5 reference(s)