Tg(KRT5-Trp63_iTA)2Gml
Transgene Detail

Summary

• Symbol: Tg(KRT5-Trp63_iTA)2Gml
• Name: transgene insertion 2, Gerry Melino
• MGI ID: MGI:5004702
• Synonyms: TAp63alpha transgene, Tg(KRT5-TAp63)2Gml
• Transgene: Tg(KRT5-Trp63_iTA)2Gml Location: unknown
• Alliance: Tg(KRT5-Trp63_iTA)2Gml page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(KRT5-Trp63_iTA)2Gml expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Trp63 (MGI:1330810)

• Tg(KRT5-Trp63_iTA)2Gml expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  cattle	KRT5 (281268)

• Mutation details: This transgene contains the bovine keratin 5 promoter driving expression of a cDNA encoding the long isoform of mouse transformation related protein 63, which includes the amino-terminal transactivation domain ("TAp63"), with a hemagglutinin (HA) tag at its amino terminus. Immunoblot analysis using antibodies vs. either TRP63 or HA demonstrates expression specifically in tissues where keratin 5 normally is expressed, including eye, lung, thymus and the basal layer of the epidermis. Immunostaining of skin biopsies and of cultured primary keratinocytes from transgenic mice showed that the transgene is overexpressed as a nuclear protein in cells of the epidermal basal layer. (J:126411)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two founder mice were used to generate mouse lines, which exhibited similar levels of expression of the transgene-encoded protein in the basal epithelial layer. No distinction was made between the lines. (J:126411)

References

• Original: J:126411 Candi E, et al., Differential roles of p63 isoforms in epidermal development: selective genetic complementation in p63 null mice. Cell Death Differ. 2006 Jun;13(6):1037-47
• All: 3 reference(s)