Tg(Thy1-COP3/EYFP)1Gfng
Transgene Detail

Summary

• Symbol: Tg(Thy1-COP3/EYFP)1Gfng
• Name: transgene insertion 1, Guoping Feng
• MGI ID: MGI:4454790
• Synonyms: Thy1-vChR1-EYFP Line 1, Thy1-vChR1-YFP line 1
• Transgene: Tg(Thy1-COP3/EYFP)1Gfng Location: unknown
• Alliance: Tg(Thy1-COP3/EYFP)1Gfng page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A fusion protein was created with a synthetic, mammalian codon-optimized, red-shifted channelrhodopsin-1 cDNA sequence derived from Volvox carteri (VChR1; GenBank accession number EU622855 [residues 1-300]) fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence (EYFP). This enhanced fusion protein (VChR1::YFP) was placed downstream of the modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns), and followed by a bovine growth hormone polyA signal. Mice derived from founder line 1 exhibit medium and sparse labeling of EGFP at cortex, CA1, CA3 and dentate gyrus of hippocampus, thalamus, superior colliculus, inferior culliculus, brainstem, amydagala. (J:160658)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:160658 Feng G, DDS for Tg(Thy1-COP3/EYFP)1Gfng. MGI Direct Data Submission. 2010
• All: 2 reference(s)