Tg(TcraKB5.C20,TcrbKB5.C20)10395Arnd
Transgene Detail

Summary

• Symbol: Tg(TcraKB5.C20,TcrbKB5.C20)10395Arnd
• Name: transgene insertion 10395, Bernd Arnold
• MGI ID: MGI:3829598
• Synonyms: Des-TCR, Des.TCR, KB5.C20, Tg(KB5.C20-TCR)
• Transgene: Tg(TcraKB5.C20,TcrbKB5.C20)10395Arnd Location: unknown
• Alliance: Tg(TcraKB5.C20,TcrbKB5.C20)10395Arnd page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraKB5.C20,TcrbKB5.C20)10395Arnd expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: The rearranged Tcra and Tcrb genes isolated from the CTL clone KB5.C20 of Bl O.BR (H-2k^b) origin were isolated. After insertion of the TCR B enhancer between the rearranged VB and CB sequences, the alpha and beta TCR transgene constructs were coinjected. Ten founders were obtained, which had integrated several copies of both transgenes as judged by Southern blot analysis with alpha and beta chain-specific probes. This line, 10395, show a high percentage of thymocytes that express the clonotype. (J:28590, J:134775)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Mice carrying this transgene express a T cell receptor (TCR) specific for the mouse MHC class I molecule H-2K^b and self peptide.

References

• Original: J:134775 Schonrich G, et al., Down-regulation of T cell receptors on self-reactive T cells as a novel mechanism for extrathymic tolerance induction. Cell. 1991 Apr 19;65(2):293-304
• All: 11 reference(s)