Tg(CAG-luc,-GFP)L2G85Chco
Transgene Detail

Summary

• Symbol: Tg(CAG-luc,-GFP)L2G85Chco
• Name: transgene insertion L2G85, Christopher H Contag
• MGI ID: MGI:3844218
• Synonyms: L2685, L2G85, luc⁺, Tg (CAG- Luc, GFP)
• Transgene: Tg(CAG-luc,-GFP)L2G85Chco Location: unknown Genetic Position: Chr7, Syntenic
• Alliance: Tg(CAG-luc,-GFP)L2G85Chco page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Tg(CAG-luc,-GFP)L2G85Chco involves 1 genes/genome features (Ptprh) View all
• Mutation details: A transgenic construct containing a modified firefly luciferase gene open reading frame, 54bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site) and eGFP sequence under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer (CAG), was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. (J:137739, J:148234)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CAG-luc,-GFP)L2G85Chco

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available

Notes

Fine mapping at The Jackson Laboratory indicates that the transgene inserted into Ptprh exon 10 [Chr7:4,558,956-4,569,937 ; mm10]. Luciferase ddPCR performed on homozygous mice (n=17) showed ~11-19 copies of the transgene. Western blot of intestinal tissue from homozygous mice (n=6) showed no PTPRH expression.

References

• Original: J:148234 Cao YA, et al., Shifting foci of hematopoiesis during reconstitution from single stem cells. Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):221-6
• All: 51 reference(s)