Tg(Acrv1-EGFP)2727Redd
Transgene Detail

Summary

• Symbol: Tg(Acrv1-EGFP)2727Redd
• Name: transgene insertion 2727, P Prabhakara Reddi
• MGI ID: MGI:3698316
• Synonyms: -408SP10-gfp, SP10-GFP, SP10-GFP line 2727
• Transgene: Tg(Acrv1-EGFP)2727Redd Location: unknown
• Alliance: Tg(Acrv1-EGFP)2727Redd page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This transgene contains a cDNA encoding enhanced green fluorescent protein, with an SV40 polyadenylation signal, downstream of a promoter fragment from the mouse acrosomal vesicle protein 1 gene encompassing nucleotides -408 through +28 (relative to the transcription start site). 21-day-old, but not 18-day-old male transgenic mice exhibit EGFP fluorescence specifically in seminiferous tubules, initially in postmeiotic, round spermatids. (J:58301)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two of three transgenic founder mice expressed EGFP in testis only; the third failed to express it in any tissue. No distinction was made between the two expressing lines.

References

• Original: J:58301 Reddi PP, et al., Round spermatid-specific transcription of the mouse SP-10 gene is mediated by a 294-base pair proximal promoter. Biol Reprod. 1999 Nov;61(5):1256-66
• All: 1 reference(s)