Tg(Itgax-cre/ERT)61Gkl
Transgene Detail

Summary

• Symbol: Tg(Itgax-cre/ERT)61Gkl
• Name: transgene insertion 61, George Kollias
• MGI ID: MGI:3690924
• Synonyms: CD11cCreERT, Tg(CD11c-CreERt)61Gkl, Tg(Itgax-cre/ESR1)61Gkl
• Transgene: Tg(Itgax-cre/ERT)61Gkl Location: unknown
• Alliance: Tg(Itgax-cre/ERT)61Gkl page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Itgax-cre/ERT)61Gkl expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Itgax (MGI:96609)

• Mutation details: The protein product of this transgene, whose transcription is under control of the dendritic cell- (DC-) specific mouse integrin alpha X (Itgax/Cdc11c) promoter, is a fusion of Cre recombinase and a mutant hormone-binding domain from human estrogen receptor 1 (ESR1). Following TAM treatment of mice bearing both this transgene and an EGFP-expressing Cre recombinase reporter transgene, green fluorescence was observed in 4-8% of CD11c⁺ cells in the spleen, lymph nodes, and thymus, but in no CD11c^- cells. Staining of Cdc11c⁺ splenocytes from these mice with CD8alpha-APC/CD-PE 40 hours after TAM administration revealed fluorescence of all major classes of DC. (J:114742)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Itgax (mouse) Summary of all recombinase alleles driven by Itgax. Alliance mouse Itgax gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:114742 Probst HC, et al., Inducible transgenic mice reveal resting dendritic cells as potent inducers of CD8+ T cell tolerance. Immunity. 2003 May;18(5):713-20
• All: 7 reference(s)