Tg(Lck-Egr1)40Tmiy
Transgene Detail

Summary

• Symbol: Tg(Lck-Egr1)40Tmiy
• Name: transgene insertion 40, Toru Miyazaki
• MGI ID: MGI:3653836
• Synonyms: Egr-1 Tg, Egr-1-TG, Egr-TG
• Transgene: Tg(Lck-Egr1)40Tmiy Location: unknown
• Alliance: Tg(Lck-Egr1)40Tmiy page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Lck-Egr1)40Tmiy expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Egr1 (MGI:95295)

• Mutation details: A 2.3-kb genomic DNA fragment containing all coding exons and intron 1, but no 3' noncoding sequence, of the 129X1/SvJ mouse early growth response 1 gene was inserted into an expression cassette containing 3.2 kb of 5' proximal sequence from the mouse lymphocyte protein tyrosine kinase gene and the polyadenylation signal and 3'untranslated region from the human growth hormone gene. This promoter directs expression primarily to immature thymocytes. Northern blot analysis demonstrated much higher than endogenous expression in thymi of transgenic mice, but detected no transgene-derived mRNA in spleen or lymph nodes. (J:111094)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This is one of three lines derived from independent transgenic founder mice.

References

• Original: J:111094 Miyazaki T, Two distinct steps during thymocyte maturation from CD4-CD8- to CD4+CD8+ distinguished in the early growth response (Egr)-1 transgenic mice with a recombinase-activating gene-deficient background. J Exp Med. 1997 Sep 15;186(6):877-85
• All: 6 reference(s)