Tg(DRE-lacZ)2Gswz
Transgene Detail

Summary

• Symbol: Tg(DRE-lacZ)2Gswz
• Name: transgene insertion 2, Thomas A Gasiewicz
• MGI ID: MGI:3629068
• Synonyms: line B
• Transgene: Tg(DRE-lacZ)2Gswz Location: unknown
• Alliance: Tg(DRE-lacZ)2Gswz page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene comprises two dioxin-responsive elements (DRE) and the minimal TATA box of the chicken ovalbumin gene upstream of the bacterial lacZ gene, which is followed by an SV40 intron and polyadenylation signal. In the absence of induction by an aryl hydrocarbon receptor (AhR) agonist, weak beta-galactosidase activity is observed only in the embryonic genital tubercle at gestational day E13. (J:109506, J:109507)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Of transgenic lines derived from 7 founder mice that transmitted the transgene, only the present line - designated line B by the authors - exhibited significant hepatic beta-galactosidase induction by TCDD (~20-fold greater than in vehicle-treated mice).

Following exposure of the adult or fetal transgenic mouse to xenobiotic ligands, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorinated byphenyls (PCBs), lacZ expression is induced in multiple target tissues. Mice bearing this transgene may be useful for investigations of toxicology, teratogenic and xenobiotic processes, Per-Arnt-Sim transcription factors, and the temporal and spatial context of transcriptionally active aryl hydrocarbon receptor (AhR) following agonist exposure in vivo.

References

• Original: J:109506 Willey JJ, et al., Aryl hydrocarbon receptor activation in genital tubercle, palate, and other embryonic tissues in 2,3,7, 8-tetrachlorodibenzo-p-dioxin-responsive lacZ mice. Toxicol Appl Pharmacol. 1998 Jul;151(1):33-44
• All: 3 reference(s)