Tg(HK2-luc)279Uku
Transgene Detail

Summary

• Symbol: Tg(HK2-luc)279Uku
• Name: transgene insertion 279, University of Kuopio
• MGI ID: MGI:3522710
• Synonyms: Uku279
• Transgene: Tg(HK2-luc)279Uku Location: unknown
• Alliance: Tg(HK2-luc)279Uku page

Transgene origin

• Strain of Origin: (BALB/c x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This transgene comprises 4077 base pairs of 5' flanking DNA and 106 base pairs of untranslated sequence from exon I of the human hexokinase II gene joined to the firefly luciferase coding sequence, which is followed by the SV40 small intron and polyadenylation signal. (J:93560, J:95065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

No distinction was made among 7 transgenic lines - this (which is not mentioned in the primary reference) and Tg(HK2-luc)255Uku, 256Uku, 257Uku, 261Uku, 278Uku and 280Uku - carrying from 1-2 to 85-95 copies of the same construct; the authors state there were no significant differences among them, whether or not results were normalized to copy number. It was noted that baseline luciferase expression varied inversely with transgene copy number. Mean basal luciferase activities in heart, skeletal muscle and adipose tissue of 4 to 5-week old transgenic mice were barely above background and were 4- to 26-fold and 8- to 77-fold lower than those of mice bearing transgenes containing 505 and 819 base pairs, respectively, of the H2K promoter. Insulin induction in 5 to 6-week old transgenic mice by 4 consecutive glucose injections did not significantly increase luciferase activity in heart or skeletal muscle.

References

• Original: J:93560 Pirinen E, et al., Analysis of the human hexokinase II promoter in vivo: lack of insulin response within 4.0 kb. Biochim Biophys Acta. 2004 Jan 20;1676(2):149-54
• All: 2 reference(s)