Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  1
• Reference
  J:158495 Burrage LC, et al., Genetic resistance to diet-induced obesity in chromosome substitution strains of mice. Mamm Genome. 2010 Apr;21(3-4):115-29
• ID
  MGI:5779641

Genes

Gene	Assay Type
Obrq18	resistance/susceptibility
Obrq19	resistance/susceptibility

Notes

• Experiment
  The C57BL/6J-Chr^A/J/NaJ (B6.A) panel of chromosome substutution strains (CSSs) is a unique model for studying resistence to diet induced obesity. C57BL/6J male mice are significantly more susceptible than A/J males to diet induced obesity. In the present study 20 CSS intercrosses, one derived from each of the 19 autosomes and chromosome X, were used to determine the number and location of quantitative trait loci (QTL) on individual chromosomes.
  
  CSS females were crossed with C57BL/B6 (B6) males), F1 offspring were then intercrossed to generate F2 male progeny, 75-93 per CSS cross. QTL analysis on the intercosses between each CSS strain (except CSS-Y and CSS-Mito) and the B6 host strain revealed significant QTL on Chromosomes 1, 6, 10, 11, and 13. The analysis was performed testing for QTL that affected:
  IW, weight in grams at app 35 days of age;
  MW, weight in grams at app 90 days of age;
  FW, weight in grams at app 135 days of age;
  BMI, FW in grams / final nasonanal length in cms;
  EWG, mean weight gain per day in grams/day for app first 55 days;
  FWG, mean weight gain per day in grams per day for second app 45 days;
  WG, mean weight gain per day in grams/per day.
  
  On Chromosome 1 (C57BL/6J-Chr 1^A/J/NaJ x C57BL/6J) the following significant QTL were identified:
  Obrq18 (obesity resistance QTL 18) mapped to Chr 1 at 67 cM in a 1.5 LOD support interval between 57 and 69 cM with a LOD=4.88, p=0.024 for trait BMI; and
  Obrq19 (obesity resistance QTL 19) at 60 cM in a 1.5 LOD support interval between 47 and 69 cM with a LOD=4.66, p=0.036 for trait WG.
  
  Curator Note: Table 3 equated these two QTL (Obrq18, Obrq19) with existing QTL Obrq4. Because Obrq4 was orginally mapped to Chr 6 in J:142667 using a mapping population between CSS-6 and C57BL/6J, which differs from the mapping population used here, we consider the current study a a separate mapping experiment and have named the QTL Obrq18 and Obrq19, respectively.
  
  The following suggestive QTL were also identified on Chr 1:
  at 62 cM, spanning 47-69 cM, LOD=4.46, p=0.054 affecting trait FW; and
  at 65 cM, spanning 37-69 cM, LOD=3.56, p=0.29 affecting trait MW.
  
  [Table 2 and Sup Table 5].