Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  8
• Reference
  J:215970 Recla JM, et al., Precise genetic mapping and integrative bioinformatics in Diversity Outbred mice reveals Hydin as a novel pain gene. Mamm Genome. 2014 Jun;25(5-6):211-22
• ID
  MGI:5693638

Genes

Gene	Assay Type
Tpnr6	visible phenotype
Hydin

Notes

• Reference
  The Diversity Outbred heterogeneous stock (J:DO) is a developing mouse population derived from progenitor lines of the Collaborative Cross (CC). The CC is a panel of recombinant inbred (RI) mouse strains that combines the genomes of eight genetically diverse founder strains - A/J, C57BL/6J, 129S1/SvImJ, NOD/ShiLtJ, NZO/HlLtJ, CAST/EiJ, PWK/PhJ, and WSB/EiJ - to capture nearly 90% of the known variation present in laboratory mice (Churchill et al. 2004). Animals from 160 incipient CC lines at early stages of inbreeding were used to establish the DO population, which is maintained by a randomized outbreeding strategy that avoids brother-sister matings. The DO and CC populations thus capture the same set of natural allelic variants derived from a common set of eight founder strains, with DO mice being outbred and the CC population being inbred.
  
  CTC (2004), Churchill, G. A., et al.. The Collaborative Cross, a community resource for the genetic analysis of complex traits. Nat Genet. 36, 1133-7.
  
  
• Experiment
  Linkage analysis was performed on 261 male and female J:DO mice (from generations G4 and G5) using 7,851 SNP markers to identify QTL associated with acute thermal pain sensitivity (hot plate test). All coordinates are relative to GRCm38.
  
  QTL Tpnr6 maps to 110.44 - 114.23 Mb (UNC080383989 - rs31329971) on Chromosome 8 with a peak LOD score of 6.1 at 113.02 Mb (rs31242180) in linkage with hot plate sensitivity. A/J alleles confer increased susceptibility to acute thermal pain; C57BL/6J alleles confer decreased susceptibility to acute thermal pain. The protein-coding gene Hydin is reported as a candidate gene.