Mapping Data

Experiment

• Experiment
  TEXT-Meta Analysis
• Chromosome
  12
• Reference
  J:123776 Wergedal JE, et al., Mapping genetic loci that regulate lipid levels in a NZB/B1NJxRF/J intercross and a combined intercross involving NZB/B1NJ, RF/J, MRL/MpJ, and SJL/J mouse strains. J Lipid Res. 2007 Aug;48(8):1724-34
• ID
  MGI:5617988

Genes

Gene
Chldq14
Tglq5
Tglq6

Notes

• Experiment
  Linkage analysis was performed on 542 female (NZB/BlNJ x RF/J)F2 intercross animals to identify QTLs associated with serum lipid levels. F2 female animals were fed a CHOW diet and sacrificed at 10 weeks of age for phenotype analysis. A panel of 94 polymorphic markers was used for linkage analysis. Parental strain NZB/BlNJ displays 48% increased plasma cholesterol and 57% increased plasma HDL compared to RF/J, and parental strain RF/J has 58% increased triglycerides and 10% higher body weight compared to NZB/BlNJ.
  
  Data from a previously studied population of 621 (MRL/MpJ x SJL/J)F2 females was also used for a combined analysis. A set of 231 microsatellite markers was used to genotype the combined data set.
  
  In the combined cross analysis [Table. 2.]:
  
  A QTL on mouse Chromosome 12, Chldq14 (cholesterol and HDL QTL 14) was detected in the combined dataset with LOD=5.98 for plasma cholesterol at 22 cM and LOD=4.51 for plasma HDL at 8.0 cM.
  
  Triglyceride QTL Tglq4, identified in the initial (NZB/BlNJ x RF/J)F2 cross, was resolved into two distinct peaks in the combined dataset.
  
  The proximal QTL, named Tglq5 (triglyceride QTL 5), mapped from 0 cM - 12 cM with the peak at D12Mit182, LOD=10.14. Fig.7.
  Apob (2 cM) and Lpin1 (9 cM) are potential candidates forTglq5.
  
  The distal QTL, named Tglq6 (triglyceride QTL 4b), mapped from 29 cM - 34 cM with peak linkage at D12Mit201, LOD=7.75. Fig.7.
  Pigh (29 cM) is a potential candidate gene for Tglq6. Ascla6 (24 cM) is an atherosclerosis QTL mapping near Tglq6.