Mapping Data

Experiment

• Experiment
  TEXT-Cytogenetic Localization
• Chromosome
  Y
• Reference
  J:21470 King TR, et al., Deletion mapping by immunoselection against the H-Y histocompatibility antigen further resolves the Sxra region of the mouse Y chromosome and reveals complexity of the Hya locus. Genomics. 1994 Nov 1;24(1):159-68
• ID
  MGI:47148

Genes

Gene	Assay Type	Description
Uba1y-rs1	Southern analysis	pUbe1y1
DYBish9	Southern analysis	B3
Sry	PCR amplified length variant	SryF, ,SryR
DYBish2	Southern analysis	pSx1
DYBish3	Southern analysis	pSx1
DYBish4	Southern analysis	pSx1
DYBish20	Southern analysis	D3-1
Uba1y-rs2	Southern analysis	pUbe1y1
DYBish10	Southern analysis	pT5.1.1.PH
DYBish14	Southern analysis	pZfB.21.A2
DYBish17	Southern analysis	pZfB.21.A2
Uba1y-rs3	Southern analysis	pUbe1y1
DYBish11	Southern analysis	PH-5
DYBish15	Southern analysis	pZfB.21.A2
Hykk2	immunological
Zfy2	PCR amplified length variant	Zfy-pA, Zfy-pB
DYBish21	Southern analysis	pT5.1.1.PH
DYBish13	Southern analysis	pZfB.21.A2
DYBish16	Southern analysis	pZfB.21.A2
DYBish6	Southern analysis	pSx1
Hyab	immunological
Uty	immunological
Hykk1	immunological
Hydk	immunological
Uba1y	Southern analysis	pUeb1y1
Zfy1	PCR amplified length variant	Zfy-pA, Zfy-pB
Uba1y-rs4	Southern analysis	pUeb1y1
DYBish24	Southern analysis	Y207
Bkm1	Southern analysis	pErs5A-4/685

Notes

• Experiment
  173 cytotoxic T-lymphocytes (CTL) which selectively express H-Y antigens were employed in the selection of somatic cells which carry deletions within the region of the Hya locus on the mouse Y Chromosome. These somatic cells were identified to facilitatemapping of loci within the Sxr^a region of the mouse Y-Chromosome. All of the H-Y deletions expressed Amel. Most of the lines were negative for Sry, Zfy1, Zfy2, Bkm1 and Mov15, therefore the variant deletion mutants expressing these loci were an asset in mapping the region. The following order was determined from the deletion mapping: Amelx - Mov15 - Ube1y1-rs1 - DYBish9 - Sry - DYBish2 - DYBish3 - DYBish4 - DYBish20 - Ube1y1-rs2 - DYBish10 - DYBish14 - DYBish17 - Ube1y1-rs3 - DYBish11 - DYBish15 - Zfy2- DYBish21 - DYBish13 - DYBish16 - Ube1y1 - Zfy1 - Ube1y1-rs4 - DYBish24. Using these cells the authors also show that the mouse Hya locus which is located within the Sxr^b deletion can be separated in 4 H-Y coding loci namely, Hyab, Hydb, Hykk1 anf Hydlk. In addition to the 4 Hya region loci a fourth locus Hykk2 is indicated as mapping proximal to Sxr^b.