Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  19
• Reference
  J:20807 Keller SA, et al., Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics. 1994 Sep 15;23(2):309-20
• ID
  MGI:47051

Genes

Gene	Assay Type	Description
D19Mit11.1	PCR amplified length variant
D19Mit17	PCR amplified length variant
D19Mit24	PCR amplified length variant
D19Mit27	PCR amplified length variant
Scd1	Southern analysis	pAL122
Dntt	Southern analysis	Tdt-1
Cyp2c29	Southern analysis	pM8-1
Adrb1	Southern analysis	[b]-1
D19Mit1	PCR amplified length variant
D19Mit3	PCR amplified length variant
D19Mit4	PCR amplified length variant
D19Mit5	PCR amplified length variant
D19Mit9	PCR amplified length variant
D19Mit10	PCR amplified length variant
D19Mit19	PCR amplified length variant
D19Nds1	reported elsewhere
Del(19)Tg8052Mm	Southern analysis	Del(19)TgN8052Mm clone 1
Krd	visible phenotype

Notes

• Reference
  Authors use Scd-1, Adrb-1 and Cyp2c for locus symbols Scd1, Adrb1 and Cyp2c29 respectively.
• Experiment
  To estimate the size of the chromosomal deletion associated with the transgene Del(19)TgN8052Mm in strain ((Krd/+ x SPRET/Ei)F1 x C57BL/6J)), PCR was applied to access the presence or absence of a number of MIT markers. It was found that SPRET/Ei alleles but not C3H/He or C57BL/6 alleles were present for D19Mit11, D19Mit17, D19Mit24, D19Mit27 and D19Nds1 indicating that these microsatellite loci were deleted in the transgenic chromosome. Deletion of Scd1 was also confirmed by Southern. Dntt, Cyp2c29, Adrb1, Cyp17 (tested by Southern) and D19Mit1, D19Mit3, D19Mit4, D19Mit5, D19Mit9, D19Mit10 and D19Mit19 (tested by PCR) were present in the transgenics. The Krd phenotype is deleted by Del(19)TgN8052Mm