Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  6
• Reference
  J:32009 Innis JW, et al., Orientation of the Hoxa complex and placement of the Hd locus distal to Hoxa2 on mouse chromosome 6. Mamm Genome. 1996 Mar;7(3):216-7
• ID
  MGI:46376

Genes

Gene	Allele	Assay Type	Description
Hoxa1		overlapping clones	P1-306, P1-4.1.1
D6Mit93		PCR amplified length variant	D975
Hoxa2		overlapping clones	P1-306, P1-4.1.1
Hoxa3		overlapping clones	P1-306, P1-4.1.1
Hoxa4		overlapping clones	P1-306, P1-4.1.1
Hoxa5		overlapping clones	P1-306, P1-4.1.1
Hoxa6		overlapping clones	P1-306, P1-4.1.1
Hoxa7		overlapping clones	P1-306, P1-4.1.1, P1-1.10
Hoxa9		overlapping clones	P1-306, P1-4.1.1
Hoxa10		overlapping clones	P1-306, P1-4.1.1
Hoxa11		overlapping clones	P1-306, P1-4.1.1
Hoxa13	Hoxa13^Hd	visible phenotype
Evx1		PCR amplified length variant

Notes

• Experiment
  The authors physically mapped the following genes on mouse Chromosome 6 using overlapping phage clones P1-Evx, P1-1.10, P1-306 and P1-4.1.1: Hoxa1, Hoxa2, Hoxa3, Hoxa4, Hoxa5, Hoxa6, Hoxa7, Hoxa9, Hoxa10, Hoxa11, Hoxa13 and Evx1. A recombinational breakpoint was determined to exist in two of the (B6C3Fe-a/a-Hd x M. spretus)F1 x B6C3Fe +/+ mice in the region between D6Mit93 (Hoxa2) and (Hoxa9). RFLP analysis of Hd using D6Mit93 PCR products was consistent with the marker being close to this mutation. The most 5' gene of the Hox cluster (Hoxa13) was suggested to possibly be the best candidate for initial screening for Hd because of reported phenotypes of Hoxa10 and Hoxa11 mice, and the more distal effects observed in Hd mice.