Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  4
• Reference
  J:26137 MacPhee M, et al., The secretory phospholipase A2 gene is a candidate for the Mom1 locus, a major modifier of ApcMin-induced intestinal neoplasia. Cell. 1995 Jun 16;81(6):957-66
• ID
  MGI:45851

Genes

Gene	Assay Type	Description
D4Mit16	PCR amplified length variant	A65
D4Mit148	PCR amplified length variant	MT173
Pla2g2a	Southern analysis	Pla2s cDNA1
D4Mit13	PCR amplified length variant	M169

Notes

• Reference
  Because the locus Pla2g2a mapped to the same region of Chromosome 4 as Mom1 the authors investigated Pla2g2a as a candidate gene for Mom1. Using Nouthern blots the authors found no expression of Pla2g2a in strains of mice susceptible for colorectal cancers, whereas resistant strains express the gene. Sequence analysis of cDNA's from both susceptible and resistant strains revealed that the Pla2g2a gene in susceptible strains contains a thymidine insert at position 207 that causes a frameshift mutation andtruncated transcript. The combined evidence suggests strongly that Pla2g2a is the candidate gene for Mom1.
• Experiment
  Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <a> <a> <a> <a>/<s> <s> <s> <s>
• Strain
  (AEJ/Gn-a Gdf5/a Gdf5 x M. spretus)F1
• Male Parent
  <a> <a> <a> <a>/<a> <a> <a> <a>
• Strain
  AEJ/Gn-a Gdf5
• Allele 1
  a from AEJ/Gn-a Gdf5
• Allele 2
  s from M. spretus

CROSS Data

MC #mice	D4Mit16	D4Mit148	Pla2g2a	D4Mit13
60	a	a	a	a
82	s	s	s	s
7	s	a	a	a
10	a	s	s	s
0	s	s	a	a
1	a	a	s	s
3	s	s	s	a
4	a	a	a	s

Additional Mice Typed Only for Specific Pairs of Loci

Marker 1	Marker 2	# Recombinants	# Parentals
D4Mit16	D4Mit148	2	14
D4Mit148	Pla2g2a	0	3
Pla2g2a	D4Mit13	1	4

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
D4Mit16	D4Mit148	19	183	10.383	2.255
D4Mit148	Pla2g2a	1	170	0.588	0.587
Pla2g2a	D4Mit13	8	172	4.651	1.606