Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  8
• Reference
  J:24375 Koizumi T, et al., Homologs of genes and anonymous loci on human chromosome 13 map to mouse chromosomes 8 and 14. Mamm Genome. 1995 Apr;6(4):263-8
• ID
  MGI:45223

Genes

Gene	Assay Type	Description
Col4a2	Southern analysis	HT39
Col4a1	Southern analysis	HT21
F10	Southern analysis	pcHX14
D8H13S35	Southern analysis	pCRI323

Notes

• Experiment
  Previous work by Kaytes et al., 1988 (J9494) indicated that Col4a2 and Col4a1 are separated by no more than 270bp. The unexpectedly high recombination frequency detected by this backcross has been interpreted by the authors in two ways: 1) The locus identified here as Col4a2 may really be a pseudogene quite distant from the real Col4a2 locus; 2) There may be a recombination hotspot between Col4a2 and Col4a1. The exact nature of this problem is currently under study.Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <d> <d> <d> <d>/<m> <m> <m> <m>
• Strain
  (DS/EiNa x MOLF/Ei)F1
• Male Parent
  <d> <d> <d> <d>/<d> <d> <d> <d>
• Strain
  C3H/HeJ
• Allele 1
  d from DS/EiNa
• Allele 2
  m from MOLF/EiJ

CROSS Data

MC #mice	Col4a2	Col4a1	F10	D8H13S35
43	d	d	d	d
34	m	m	m	m
2	m	d	m	m
0	d	m	d	d
6	m	d	d	m
1	d	m	m	d
1	m	d	d	d
4	d	m	m	m
1	m	m	m	d
1	d	d	d	m

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
Col4a2	Col4a1	14	93	15.054	3.708
Col4a1	F10	2	93	2.151	1.504
F10	D8H13S35	9	93	9.677	3.066