Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  12
• Reference
  J:19675 Jacoby RF, et al., Genetic analysis of colon cancer susceptibility in mice. Genomics. 1994 Jul 15;22(2):381-7
• ID
  MGI:44738

Genes

Gene	Assay Type	Description
D12Nds11	PCR amplified length variant
D12Mit2	PCR amplified length variant
D12Mit4	PCR amplified length variant
D12Mit5	PCR amplified length variant	L58
D12Mit51	PCR amplified length variant
Fos	PCR amplified length variant	D12Rfj1-pA, D12Rfj1-pB
D12Mit6	PCR amplified length variant	L16
Ccs1	visible phenotype
D12Mit30	PCR amplified length variant	A663
D12Mit7	PCR amplified length variant
D12Mit8	PCR amplified length variant

Notes

• Experiment
  A genome wide search was made in an attempt to find microsatellite markers linked to Ccs1 (colon cancer susceptibility 1) in 122 progeny derived from a (ICR/Ha x C57BL/6Ha)F1 x C57BL/6Ha backcross. ICR/Ha is susceptible and C57BL/6Ha resistant to DMH induced colon tumors. 177 polymorphic markers spaced 5 cM - 30 cM apart were used for the genome scan. Chi square tests indicated linkage with all tested Chromosome 12 markers and the association of Ccs1 with D12Mit6 (45 cM) showed the greatest significance (P < 0.001)> A LOD score of 2.9 between Ccs1 and D12Mit6 was also indicated. ICR/Ha-derived alleles at Ccs1 are associated with colon tumor susceptibility whereas C57BL/6Ha-derived alleles are associated with colon tumor resistance. A potential candidate gene mappingnear Ccs1 is Tgfb3 (41 cM).
  
  A linkage map of Chromosome 12 based on the backcross analysis indicated the following order: D12Nds11 - 14.5 cM - D12Mit2 - 12.9 cM - D12Mit4 - D12Mit5 - 3.2 cM - D12Mit51 - D12Rfj1 -3.2 cM - D12Mit6 - Ccs1 - 3.2 cM - D12Mit30 - 4.8 - cM - D12Mit7 - 9.7 cM - D12Mit8.