Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  18
• Reference
  J:21512 Goldstein C, et al., Of mice and Marfan: genetic linkage analyses of the fibrillin genes, Fbn1 and Fbn2, in the mouse genome. Mamm Genome. 1994 Nov;5(11):696-700
• ID
  MGI:44126

Genes

Gene	Assay Type	Description
Fgf1	Southern analysis	pJC3-5
D18Mit35	PCR amplified length variant	D648
Fbn2	Southern analysis	Fbn2 cDNA1
Pdgfrb	Southern analysis	pHH64
Mtv38	Southern analysis	8-21

Notes

• Reference
  The authors suggest that Fbn1 and Fbn2 may be candidate genes for the Tsk and pk mutations respectively.
• Experiment
  Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <a> <a> <a> <a> <a>/<s> <s> <s> <s> <s>
• Strain
  (AEJ/Gn-a Gdf5/a Gdf5 x M. spretus)F1
• Male Parent
  <a> <a> <a> <a> <a>/<a> <a> <a> <a> <a>
• Strain
  AEJ/Gn-a Gdf5
• Allele 1
  a from AEJ/Gn-a Gdf5
• Allele 2
  s from M. spretus

CROSS Data

MC #mice	Fgf1	D18Mit35	Fbn2	Pdgfrb	Mtv38
53	a	a	a	a	a
71	s	s	s	s	s
3	a	s	s	s	s
4	s	a	a	a	a
6	a	a	s	s	s
7	s	s	a	a	a
1	a	a	a	s	s
1	s	s	s	a	a
3	a	a	a	a	s
8	s	s	s	s	a

Additional Mice Typed Only for Specific Pairs of Loci

Marker 1	Marker 2	# Recombinants	# Parentals
Fgf1	D18Mit35	3	18
D18Mit35	Fbn2	1	14
Fbn2	Pdgfrb	0	10
Pdgfrb	Mtv38	1	14

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
Fgf1	D18Mit35	10	178	5.618	1.726
D18Mit35	Fbn2	14	172	8.140	2.085
Fbn2	Pdgfrb	2	167	1.198	0.842
Pdgfrb	Mtv38	12	172	6.977	1.942