Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  7
• Reference
  J:152405 Kurey I, et al., Distinct genetic control of parasite elimination, dissemination, and disease after Leishmania major infection. Immunogenetics. 2009 Sep;61(9):619-33
• ID
  MGI:4360545

Genes

Gene	Assay Type
Lmr21	resistance/susceptibility
Lmr21a	resistance/susceptibility
Lmr21b	resistance/susceptibility
D7Mit282	PCR amplified length variant
D7Mit67	PCR amplified length variant

Notes

• Experiment
  In the present study the genetics of disease symptoms, immunological parameters and parasite load in draining lymph nodes and in the spleen following Leishmania major infection were analyzed.
  
  A special tool for the genetic analysis of multigenic traits, recombinant congenic strains, were used. Each of the 20 CcS/Dem RC mouse strains carries a different random subset of 12.5% genes of the resistant donor strain STS/A (STS) on the background of the susceptible strain BALB/cHeA (BALB/c). Two hundred and ninty nine (BALB/c x CcS11/Dem)F2 mice were used (155 were females and 144 were males). The CcS11 strain was in more than 38 generations of inbreeding.
  
  The CcS11 strain differs from BALB/c at STS-derived segments on eight chromosomes. These differential segements were typed in the F2 hybrid mice between BALB/c and CcS11 using 16 mircosatellite markers with a maximal interval between any two markers of 19 cM. Linkage analysis of the F2 hybids indicated four novel loci on Chromosomes 1, 7, 12, 16, and additional effects for a previoulsy detected locus on Chromosome 10.
  
  Lmr21, leishmaniasis resistance 21, mapped to Chromosome 7 of CcS11/Dem with markers D7Mit282 at 53.5 cM and D7Mit67 at 63.5 cM.
  
  03.30.2016 Curator Note: We have assigned distinct nomenclature to the individual disease phenotypes that mapped with significance to Lmr21, creating Lmr21a and Lmr21b. Lmr21 represents the collection of individual traits analyzed that map to this same locus.
  
  Lmr21a was linked with D7Mit282, p=0.0972 and D7Mit67, p=0.0116 and influenced skin lesion size. BALB/c alleles were associated with larger skin lesions. The impact on the phenotype was higher in females than in males, linked with D7Mit67, p=0.046, in females. The QTL was not detected in male mice. [Table 4.]
  
  Lmr21b, also at D7Mit67, was detected in interaction with Lmr32e (D10Mit12) influencing IFN gamma concentrations in serum, p=0.0195 and accounting for 7.18% of trait variance.
  The lowest levels of IFN gamma were observed in either STS/A homozygotes at both loci or in BALB/c homozygotes at both loci. [Table 7.]