Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  2
• Reference
  J:14564 Siracusa LD, et al., The tight skin (Tsk) mutation in the mouse, a model for human fibrotic diseases, is tightly linked to the beta 2-microglobulin (B2m) gene on chromosome 2. Genomics. 1993 Sep;17(3):748-51
• ID
  MGI:42498

Genes

Gene	Allele	Assay Type	Description
D2Mit15		PCR amplified length variant	A61
D2Mit63		PCR amplified length variant	A1132
B2m		Southern analysis	g2B2md111B
D2Mit17		PCR amplified length variant	M246
Fbn1	Fbn1^Tsk	visible phenotype
Il1a		Southern analysis	ppIL1-1301
Bmp2		Southern analysis	Bmp2a probe
D2Mit19		PCR amplified length variant	A83
D2Mit22		PCR amplified length variant	M167

Notes

• Reference
  Authors use Bmp-2a for Bmp2.
• Experiment
  Only progeny displaying the Tsk phenotype were analyzed.Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, sexes unspecified or combined
• Female Parent
  <b> <b> <b> <b> <b> <b> <b> <b> <b>/<c> <c> <c> <c> <c> <c> <c> <c> <c>
• Strain
  (C57BL/6J-Bloc1s6 +/+ Fbn1 x M. m. castaneus)F1
• Male Parent
  <b> <b> <b> <b> <b> <b> <b> <b> <b>/<b> <b> <b> <b> <b> <b> <b> <b> <b>
• Strain
  C57BL/6J-Bloc1s6 +/+ Fbn1
• Allele 1
  b from C57BL/6J-Bloc1s6 +/+ Fbn1
• Allele 2
  c from M. m. castaneus

CROSS Data

MC #mice	D2Mit15	D2Mit63	B2m	D2Mit17	Fbn1	Il1a	Bmp2	D2Mit19	D2Mit22
70	b	b	b	b	b	b	b	b	b
12	c	b	b	b	b	b	b	b	b
1	c	c	b	b	b	b	b	b	b
2	b	b	b	b	b	c	c	c	c
1	b	b	b	b	b	b	c	c	c
5	b	b	b	b	b	b	b	b	c

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
D2Mit15	D2Mit63	12	91	13.187	3.547
D2Mit63	B2m	1	91	1.099	1.093
B2m	D2Mit17	0	91	0.000	0.000
D2Mit17	Fbn1	0	91	0.000	0.000
Fbn1	Il1a	2	91	2.198	1.537
Il1a	Bmp2	1	91	1.099	1.093
Bmp2	D2Mit19	0	91	0.000	0.000
D2Mit19	D2Mit22	5	91	5.495	2.389