Mapping Data

Experiment

• Experiment
  CROSS
• Chromosome
  6
• Reference
  J:1908 Friedman JM, et al., Molecular mapping of the mouse ob mutation. Genomics. 1991 Dec;11(4):1054-62
• ID
  MGI:40511

Genes

Gene	Allele	Assay Type	Description
Col1a2		Southern analysis	probe from Liau
Met		Southern analysis	cDNA from van de Woude
Lep	Lep^ob	visible phenotype
Cpa1		Southern analysis	probe from Quinto
Tcrb		Southern analysis	probe from Snodgrass

Notes

• Experiment
  Only ob/ob mice were scored; mice were typed for C57BL/6J vs M. spretus alleles.Offspring types indicate alleles inherited from F1 parent.
  

CROSS

• Type
  Backcross, female
• Female Parent
  <b> <b> <b> <b> <b>/<s> <s> <s> <s> <s>
• Strain
  (C57BL/6J-Lep x M. spretus)F1
• Male Parent
  <b> <b> <b> <b> <b>/<d> <d> <d> <d> <d>
• Strain
  (B6.Cg-Lep/J x DBA/2J)F1
• Allele 1
  b from C57BL/6J
• Allele 2
  s from M. spretus

CROSS Data

MC #mice	Col1a2	Met	Lep	Cpa1	Tcrb
102	b	b	b	b	b
1	s	b	b	b	b
5	s	s	b	b	b
0	s	s	s	b	b
15	s	s	s	s	b

Additional Mice Typed Only for Specific Pairs of Loci

Marker 1	Marker 2	# Recombinants	# Parentals
Met	Lep	0	4

Statistics

Marker 1	Marker 2	# Recombinants	Total	% Recombinants	Std Error
Col1a2	Met	1	123	0.813	0.810
Met	Lep	5	127	3.937	1.726
Lep	Cpa1	0	123	0.000	0.000
Cpa1	Tcrb	15	123	12.195	2.951