Mapping Data

Experiment

• Experiment
  HYBRID
• Chromosome
  X
• Reference
  J:6384 Francke U, et al., Comparative gene mapping: order of loci on the X chromosome is different in mice and humans. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3595-9
• ID
  MGI:40150

Genes

Gene	Assay Type	Description
Hprt1	electrophoretic	IEF

Notes

• Experiment
  Somatic cell hybrids were from fusion of male mouse primary fibroblasts carrying T(X;16)16H with Hprt-Chinese hamster cell line V79/380-6. Primary hybrids selected in HAT media retained both mouse 16^X and X¹⁶ translocation products and expressed bothmouse Hprt and Ags. Hybrids backselected in 8-azaguanine lost Hprt activity and lost the X¹⁶ Chromosome but retained the 16^X Chromosome and mouse Ags activity. These data localize Hprt on the X¹⁶ translocation product in the Xcen-D region and Ags on the 16^X translocation product in the XD-ter region.

HYBRID

• Concordance
  chromosomes

HYBRID Data

Chromosome	+/+	+/-	-/+	-/-
16	4	4	2	4
T(X;16	6	0	0	8