HYBRID Mapping MGI Mouse MGI:39496

Mapping Data

Experiment

HYBRID
Chromosome

2
Reference

J:10302 Brilliant MH, et al., Sequences homologous to glutamic acid decarboxylase cDNA are present on mouse chromosomes 2 and 10 [published erratum appears in Genomics 1990 Apr;6(4):708]. Genomics. 1990 Jan;6(1):115-22
ID

MGI:39496

Gene	Allele	Assay Type	Description
Gad1

Experiment

Somatic cell hybrids were from fusion of mouse with Chinese hamster cells. Hybrid DNA was digested with PstI, Southern blotted and probed for Gad-1 using feline p65-Gad-16 cDNA. The segregation of a 4.0kb mouse specific fragment for Gad-1 on Chromosome 2was analyzed. Because Chromosome 11 was not present in these somatic cell hybrids, rat-mouse hybrid RTM9 containing mouse Rb(11.13) and control RTM9Bu that lost this chromosome were analyzed and were negative for mouse Gad-1 sequences. these data ruled out Chromosome 11 as the site of Gad-1 or related sequences.

HYBRID Data

Chromosome	+/+	+/-	-/+	-/-
1	8	4	5	4
2	14	0	0	8
3	5	4	6	4
4	7	1	7	7
5	2	1	11	7
6	8	2	6	6
7	13	3	1	5
8	7	0	7	8
9	6	1	7	7
10	3	0	11	8
11	0	0	11	8
12	8	4	4	4
13	9	1	4	6
14	2	1	10	7
15	10	6	0	2
16	3	1	7	7
17	11	3	3	5
18	7	1	5	7
19	7	1	6	7
X	9	3	5	5

Chromosome	+/+	+/-	-/+	-/-
1	8	4	5	4
2	14	0	0	8
3	5	4	6	4
4	7	1	7	7
5	2	1	11	7
6	8	2	6	6
7	13	3	1	5
8	7	0	7	8
9	6	1	7	7
10	3	0	11	8
11	0	0	11	8
12	8	4	4	4
13	9	1	4	6
14	2	1	10	7
15	10	6	0	2
16	3	1	7	7
17	11	3	3	5
18	7	1	5	7
19	7	1	6	7
X	9	3	5	5

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Chromosome	+/+	+/-	-/+	-/-
1	8	4	5	4
2	14	0	0	8
3	5	4	6	4
4	7	1	7	7
5	2	1	11	7
6	8	2	6	6
7	13	3	1	5
8	7	0	7	8
9	6	1	7	7
10	3	0	11	8
11	0	0	11	8
12	8	4	4	4
13	9	1	4	6
14	2	1	10	7
15	10	6	0	2
16	3	1	7	7
17	11	3	3	5
18	7	1	5	7
19	7	1	6	7
X	9	3	5	5

Chromosome	+/+	+/-	-/+	-/-
1	8	4	5	4
2	14	0	0	8
3	5	4	6	4
4	7	1	7	7
5	2	1	11	7
6	8	2	6	6
7	13	3	1	5
8	7	0	7	8
9	6	1	7	7
10	3	0	11	8
11	0	0	11	8
12	8	4	4	4
13	9	1	4	6
14	2	1	10	7
15	10	6	0	2
16	3	1	7	7
17	11	3	3	5
18	7	1	5	7
19	7	1	6	7
X	9	3	5	5