Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  12
• Reference
  J:142770 Stylianou IM, et al., Differences in DBA/1J and DBA/2J reveal lipid QTL genes. J Lipid Res. 2008 Nov;49(11):2402-13
• ID
  MGI:3828426

Genes

Gene	Assay Type
Tgq23	visible phenotype
Hdlq63	visible phenotype

Notes

• Experiment
  Linkage analysis was performed using a backcross derived from closely related inbred strains DBA/1J and DBA/2J. The genetic composition of these 2 inbred strains differs by just 5.6% at the SNP level. A total of 294 mice from (DBA/1J x DBA/2J)F1 x DBA/1J and (DBA/1J x DBA/2J)F1 x DBA/2J populations were screened for 6,949 polymorphic SNP markers. Backcross mice were placed on a high-fat diet at 8 weeks of age and phenotyped for plasma triglyceride and HDL concentrations at 8, 11 and 16 weeks of age. Parental strain DBA/2J displays significantly increased plasma triglyceride concentrations on a CHOW diet compared to parental strain DBA/1J. Male DBA/2J animals display significantly increased plasma triglyceride and HDL concentrations after an 8 week high fat diet compared to DBA/1J males.
  
  Tgq23 (triglyceride QTL 23) mapped to 45 cM on mouse Chromosome 12 at peak marker rs3711162 (90.38 Mb) with LOD=5.6 for plasma triglyceride concentrations on a CHOW diet. DBA/2J-derived alleles at Tgq23 confer increased triglyceride levels with a stronger effect in males compared to females. Tgq23 also displays suggestive linkage to plasma triglycerides after 3 weeks on a high fat diet (LOD=3.6) and after 8 weeks on a high fat diet (LOD=3.7). [Table 2 and 3].
  
  Suggestive linkage to HDL cholesterol concentration after 3 and 8 weeks on a high fat diet (LOD=2.5) also mapped to 45 cM and is named Hdlq63 (HDL QTL 63).