Experiment
Mapping analyis of over 200 pedigrees identified three Sox10 modifiers , named modifier of Sox10 (Mos1, Mos2 and Mos3). Linkage indicated a locations on mouse Chromosomes 13, 4 and 3, respectively, For mapping of Gli3 Mos1, nine mice were
used for an initial genome scan (six affected Sox10LacZ/+; Mos1/+ mice and three
Mos1/+ heterozygous carriers). Typing of 220 SNPs with alleles in
C57BL/6J and BALB/cJ identified a 20 Mb region of proximal mouse Chr 13 where all
nine animals had inherited the BALB/cJ allele from the affected founder animal,
providing evidence for linkage to markers in this region including Gli3 wherein Mos1 was determined to be allelic.
Mos2 and Mos3 were mapped to mouse Chromosome 4 and 3, respectively. With Mos2, the authors detected linkage to mouse Chromsome 4 by utilizing 91 SSLP
markers to genotype eight affected animals. Subsequent genotyping of affected animals
(N=31) in this region localized Mos2 to 35 Mb region of Chr 4 flanked by markers
D4Mit9 (1/31 recombinant;P< 0.0001) and D4Mit203 (8/31 recombinant; P < 0.01). For
Mos3, genotyping 408 SNPs in five affected animals showed linkage
to two regions of the genome. Subsequent genotyping of SSLPs spanning those regions in
additional affected animals (N=32) refinedthe linkage to a 20 Mb region of mouse Chromosome 3 flanked by markers D3Mit178 (2/32 recombinant; P < 0.001) and D3Mit65 (3/32 recombinant; P
< 0.0001).
Analysis Tools