Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  5
• Reference
  J:129499 Ferraro TN, et al., Quantitative trait locus for seizure susceptibility on mouse chromosome 5 confirmed with reciprocal congenic strains. Physiol Genomics. 2007 Nov 14;31(3):458-62
• ID
  MGI:3770303

Genes

Gene	Assay Type
Szs11	resistance/susceptibility
D5Mit75	PCR
D5Mit403	PCR
Gabra2	reported elsewhere
Gabra4	reported elsewhere
Gabrb1	reported elsewhere
Gabrg1	reported elsewhere
Slc34a2	reported elsewhere
Cckar	reported elsewhere
Txk	reported elsewhere
Chrna9	reported elsewhere
Cnga1	reported elsewhere
Gnrhr	reported elsewhere
Grk3	reported elsewhere

Notes

• Experiment
  Previously identified QTL Szs11 (seizure susceptibility 11) on mouse Chromosome 5 was confirmed and refined using congenic line analysis. A congenic line (B6.D2-Szs11) carrying the Szs11 locus derived from DBA/2J (seizure susceptible) on a C57BL/6J (seizure resistant) genetic background was constructed. The DBA/2J donor region spans markers D5Mit75 (20 cM) and D5Mit403 (61 cM). A reciprocal congenic line was constructed with a larger C57BL/6J donor segment, extending from D5Mit345 (centromere) to D5Mit403 (56 cM). Animals were tested daily to achieve maximal electroshock seizure threshold (MEST) beginning at 8-9 weeks of age.
  
  Szs11 explained approximately 10% of the variance in congenic animals. Seizure resistance is conferred by C57BL/6J alleles at Szs11. As expected, D2.B6-Szs11 congenic animals displayed significantly higher MEST values compared to DBA/2J control animals. Conversely, B6.D2-Szs11 male congenic animals displayed lower MEST values (seizure susceptibility) compared to C57BL/6J control males. Seizure susceptibility did not differ significantly between B6.D2-Szs11 and C57BL/6J females.
  
  Obvious candidate genes mapping to the Szs11 interval include Gabra2 (40 cM), Gabra4, Gabrb1, and Gabrg1 (40 cM). However, SNP analysis did not reveal polymorphism in gene sequences between C57BL/6J and DBA/2J. Differential gene expression analysis was not performed so it is unknown if the level of mRNA transcript differs between C57BL/6J and DBA/2J. Additional candidate genes for Szs11 are Slc34a2 (31 cM), Cckar (34 cM), Txk (40 cM), Chrna9 (41 cM), Cnga1 (41 cM), Gnrhr (44 cM), and Adrbk2 (60 cM).