Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  5
• Reference
  J:125557 Santiago-Raber ML, et al., Characterization of reciprocal Lmb1-4 interval MRL-Faslpr and C57BL/6-Faslpr congenic mice reveals significant effects from Lmb3. J Immunol. 2007 Jun 15;178(12):8195-202
• ID
  MGI:3762856

Genes

Gene	Assay Type
Lmb2	resistance/susceptibility

Notes

• Experiment
  Previously identified lupus susceptibility QTLs Lmb1-Lmb4 were further studied using subcongenic line analysis. Reciprocal congenics were derived from parental strains MRL-Fas^lpr (lupus susceptible) and C57BL/6-Fas^lpr (lupus resistant).
  
  Lmb1 maps to57.5 cM on mouse Chromosome 4. B6.MRL-Lmb1 Fas^lpr congenic animals displayed increased lymph node weight while MRL.B6-Lmb1 Fas^lpr reciprocal congenic animals displayed increased spleen weight and increased CD8+ and CD4-CD8- T cell percentages.
  
  Lmb2 maps to 41 cM on mouse Chromosome 5. B6.MRL-Lmb2 Fas^lpr congenics displayed reduced CD4-CD8- splenic T cell percentages compared to background strain C57BL/6-Fas^lpr.
  
  The Lmb3 locus at 28 cM on mouse chromosome 7 displayed a major effect on lupus susceptibility. B6.MRL-Lmb3 Fas^lpr congenic animals displayed significantly larger spleens and lymph nodes compared to background strain C57BL/6-Fas^lpr. Conversely, reciprocal congenic MRL.B6-Lmb3 Fas^lpr displayed smaller spleens and lymph nodes, reducedimmune complex glomerulonephritis, and reduced IgG autoantibodies compared to B6.MRL-Lmb3 Fas^lpr. Thus, it appears Lmb3 has a significant effect on promoting spontaneous autoimmunity. Generation of subcongenic lines localized Lmb3 to an interval between D7Mit350 (41 cM, 84 Mb) and D7Mit109 (66 cM, 136 Mb). Aem2, an antibody modifier QTL at 37 cM, maps near Lmb3. Myci1,a myocardial infarction QTL, maps to 69 cM.
  
  Lmb4 maps to 41 cM on mouse Chromosome 10. The B6.MRL-Lmb4 Fas^lpr congenic displayed slightly larger lymph nodes compared to background strain C57BL/6-Fas^lpr.