Mapping Data

Experiment

• Experiment
  TEXT-Meta Analysis
• Chromosome
  7
• Reference
  J:123776 Wergedal JE, et al., Mapping genetic loci that regulate lipid levels in a NZB/B1NJxRF/J intercross and a combined intercross involving NZB/B1NJ, RF/J, MRL/MpJ, and SJL/J mouse strains. J Lipid Res. 2007 Aug;48(8):1724-34
• ID
  MGI:3720121

Genes

Gene	Assay Type
Chldq6	visible phenotype
Nr1h2	reported elsewhere
Pafah1b3	reported elsewhere

Notes

• Experiment
  Linkage analysis was performed on 542 female (NZB/BlNJ x RF/J)F2 intercross animals to identify QTLs associated with serum lipid levels. F2 female animals were fed a CHOW diet and sacrificed at 10 weeks of age for phenotype analysis. A panel of 94 polymorphic markers was used for linkage analysis. Parental strain NZB/BlNJ displays 48% increased plasma cholesterol and 57% increased plasma HDL compared to RF/J, and parental strain RF/J has 58% increased triglycerides and 10% higher body weight compared to NZB/BlNJ.
  
  Data from a previously studied population of 621 (MRL/MpJ x SJL/J)F2 females was also used for a combined analysis. A set of 231 microsatellite markers was used to genotype the combined data set.
  
  In the combined cross analysis [Table. 2.]:
  
  A QTL on mouse Chromome 7, Chldq6 (cholesterol and HDL QTL 6) linked to plasma cholesterol (LOD=6.18) and HDL (LOD=4.77) mapped to 12.0 cM in the combined data set.The 95% confidence interval spans 8cM - 20 cM. Nr1h2 and Pafah1b3 are potential candidategenes. Previously identified QTLs Hdl38 (16 cM), Lprq5 (15 cM), and Nhdlq6 (8 cM) overlap with Chldq6.