Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  2
• Reference
  J:117545 Farber CR, et al., Fine mapping reveals sex bias in quantitative trait Loci affecting growth, skeletal size and obesity-related traits on mouse chromosomes 2 and 11. Genetics. 2007 Jan;175(1):349-60
• ID
  MGI:3698551

Genes

Gene	Assay Type
D2Mit329	PCR amplified length variant
D2Mit457	PCR amplified length variant
Wg5	visible phenotype
D2Mit207	PCR amplified length variant
Wg6	visible phenotype
D2Mit260	PCR amplified length variant
Swq6	visible phenotype
D2Mit262	PCR amplified length variant
Kwq13	visible phenotype
D2Mit17	PCR amplified length variant
Kwq14	visible phenotype
Tailq7	visible phenotype
Bdlnq7	visible phenotype
D2Mit196	PCR amplified length variant
Fatmq1	visible phenotype
D2Mit223	PCR amplified length variant
Fatmq2	visible phenotype
Tcq1	visible phenotype
Tcq2	visible phenotype

Notes

• Experiment
  Wg2 (weight gain in high growth mice 2) and Wg4 (weight gain in high growth mice 4) were previously mapped to mouse Chromosomes 2 and 11, respectively. These loci were investigated further by construction of speed congenic lines carrying C57BL/6J-Socs2^hg-derived DNA at Wg2 or Wg4 on a CAST/EiJ genetic background. These lines are designated HG.CAST-(D2Mit329-D2Mit457) for the Wg2 congenic and HG.CAST-(D11Mit260-D11Mit255) for the Wg4 congenic. Parental strain C57BL/6J-Socs2^hg exhibits significantly increased growth trajectory after 3 weeks of age compared to CAST/EiJ. Over 700 F2 animals were produced from [C57BL/6J-Socs2^hg x HG.CAST-(D2Mit329-D2Mit457)]F2 and [C57BL/6J-Socs2^hg x HG.CAST-(D11Mit260-D11Mit255)]F2 intercrosses. Animals were analyzedat 9 weeks of age and screened for nineteen polymorphic markers on chromosome 2 (average resolution = 2.7 cM) and eleven markers on chromosome 11 (average resolution = 3 cM). Several novel body trait QTLs were identified and are described below.
  
  On mouseChromosome 2, linkage to 6-week and 9-week body weight mapped to 9 cM (112 Mb) near D2Mit207 and is named Wg5 (LOD=9 and 10.8, respectively). The Wg5 confidence interval spans 2 cM - 29.5 cM. In males, Wg6 is associated with 6-week and 9-week body weightat 27.5 cM (150 Mb) near D2Mit260 (LOD=7.5 and 6, respectively). CAST/EiJ-derived alleles at Wg5 and Wg6 confer decreased body weight.
  
  Linkage to spleen weight mapped to 30 cM near D2Mit262 (Swq6, LOD=11). CAST/EiJ-derived alleles at Swq6 confer increased spleen weight with additive inheritance in males, but with dominant inheritance in females. The Swq6 confidence interval spans 26 cM - 42 cM. Swq6 accounts for 16.7% of the variance.
  
  Linkage to kidney weight mapped to 12.5 cM near D2Mit17 (Kwq13, LOD=9.8 in males) and 41 cM (Kwq14, LOD=7.9). CAST/EiJ-derived alleles at Kwq13 confer increased kidney weight with additive inheritance in females, while heterozygous male mice have increased kidney weight. Kwq13 explains 15% of the variance and the confidence spans 8.5 cM - 13.8 cM. Kwq14 explains 12.3% of the variance.
  
  Linkage to tail length in females, Tailq7, mapped to 12.5 cM (120 Mb) near D2Mit17 (LOD=6.7). This locus explains 10.5% of the variance. CAST/EiJ-derived alleles at Tailq7 confer decreased tail length in females with an additive mode of inheritance. The Tailq7 confidence interval spans 2.5 cM - 22.5cM.
  
  Linkage to body length, Bdlnq7, mapped to 33 cM (159 Mb) near D2Mit196 (LOD=5.3). Homozygosity for CAST/EiJ-derived alleles at Bdlnq7 confersdecreased nasal-to-anal length with dominant inheritance and the effect is stronger in females. This locus explains 8.4% of the variance. The confidence interval spans 0.5 cM - 37.5 cM.
  
  Total fat pad mass, Fatmq1 and Fatmq2, mapped to 20.3 cM (LOD=8.7near D2Mit223) and 37 cM (LOD=5.3), respectively. CAST/EiJ-derived alleles at Fatmq1 confer decreased total fat mass in females with additive inheritance. Fatmq1 explains 13.5% of the variance and the confidence interval spans 9.7 cM - 25 cM. CAST/EiJ-derived alleles at Fatmq2 decrease total fat mass in both males and females. Fatmq2 locus explains 8.5% of the variance.
  
  Linkage to plasma total cholesterol, Tcq1 and Tcq2, mapped to 16.5 cM (LOD=5.8, 9.2% of variance) and 32 cM near D2Mit262 (LOD=6.8, 10.7% of variance), respectively. CAST/EiJ-derived alleles at Tcq1 and Tcq2 confer decreased plasma total cholesterol. The Tcq2 confidence interval spans 12.5 cM - 44.5 cM.