Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  10
• Reference
  J:108764 Havelkova H, et al., Genetics of susceptibility to leishmaniasis in mice: four novel loci and functional heterogeneity of gene effects. Genes Immun. 2006 Apr;7(3):220-33
• ID
  MGI:3625976

Genes

Gene	Assay Type
Lmr25b	resistance/susceptibility
D10Mit103	PCR amplified length variant
Lmr19	resistance/susceptibility
D10Mit67	PCR amplified length variant

Notes

• Experiment
  Disease phenotypes associated with Leishmania major infection were mapped in animals from (CcS-16 x BALB/cHeA)F2 and (CcS-20 x BALB/cHeA)F2 intercross populations. CcS-16 and CcS-20 are recombinant congenic (RC) strains derived from STS/A and BALB/cHeA. Animals were infected with Leishmania major after 9 weeks of age and sacrificed 8 weeks post-infection.
  
  F2 hybrids between BALB/c and CcS20/Dem and F2 hybrids between BALB/c and CcS16 were tested from clinical and immunological consequences post infection. They were genotyped with mircosatellite markers covering the STS-derived segments. Linkage analysis of differing pathological and immunological parameters indicated novel loci and novel functions for previously mapped loci.
  
  In the (BALB/c x CcS16)F2 intercross linkage mapping detected a significant interaction between markers D10Mit103 and D2Mit102.
  
  03.28.2016 Curator Note:
  The QTL mapping with marker D10Mit103 is referred to in the text as Lmr5 and the QTL mapping with D2Mit102 is referred to as Lmr14.
  However, Lmr5 was orginally mapped in J:82715 using (BALB/c x CcS5)F2 mice which differ from the mapping population used here. We have identified the QTL mapped here to D10Mit103, using BALB/c x CcS16)F2 mice as Lmr25. We have named the novel trait measured in this mapping experiment as Lmr25b.
  Lmr14 was originally mapped in J:82717 also using (BALB/c x CcS16)F2 however, since novel phenotypes were measured in the current study we have assigned novel nomenclature to each
  significant trait. We have named the QTL identified in this interaction, mapping with marker D2Mit102 as Lmr28d.
  
  Lmr25b (D10Mit103) was detected in interaction with Lmr28d (D2Mit102), p=0.0133. Homozygous STS/A alleles at both loci were associated with higher levels of IL-2. This interaction was attributed to 8.65% of trait variance. [Table 4.]
  
  Another interaction was identified in the (BALB/c x CcS16)F2 intercross bewteen Lmr19 (D10Mit67) and Lmr12 mapping with marker D16Mit126.
  
  03.28.2016 Curator Note: Lmr12 was previously mapped using the same intercross population used here in J:82717. However, since a different phenotype was measured in that study we have we have labeled the QTL identified in this interaction as Lmr12b.
  
  Lmr19 (D10Mit67) was identified in interaction with Lmr12c (D16Mit126), p=0.00142.
  A combination of homozygous BALB/c alleles at Lmr19 (D10Mit67) and homozygous STS/A alleles at Lmr12c (near D16Mit126) increase the spontaneous proliferation of spleen cells in infected mice. The Lmr19-Lmr12c interaction acounted for 8.53% of trait variance.