Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  4
• Reference
  J:99483 Srivastava AK, et al., Identification of novel genetic loci for bone size and mechanosensitivity in an ENU mutant exhibiting decreased bone size. J Bone Miner Res. 2005 Jun;20(6):1041-50
• ID
  MGI:3618843

Genes

Gene	Assay Type
917M	visible phenotype
Calb1	reported elsewhere
Igfbpl1	reported elsewhere
Casp8ap2	reported elsewhere
Gabrr1	reported elsewhere
Gabrr2	reported elsewhere
Cga	reported elsewhere
Rragd	reported elsewhere
Il11ra1	reported elsewhere
Musk	reported elsewhere

Notes

• Experiment
  Dominant ENU mutagenesis screening on the C57BL/6J background identified a male-specific reduced bone size mutant phenotype. At 16 weeks of age, males of the mutant line, designated 917M, exhibit 10%-13% reduction in total body bone area and 5%-8% reduced periosteal circumference of the tibia and femur midshaft compared to wild type C57BL/6J males. Male 917M animals also exhibit 17%-27% reduced cell proliferation and 23% increased apoptosis of periosteal osteoblasts compared to wild type C57BL/6J males. 917M females are not affected. Genetic loci involved in the mutant phenotype were mapped via a low resolution genome scan using 60 microsatellite markers (average spacing = 21 cM) and 129 (C57BL/6J-917M x C3H)F2 intercross animals.
  
  Significant linkage to bone size mapped to 10.5 cM on mouse Chromosome 4 with LOD=7.9. This locus is named Bnszq1 (bone size QTL 1). When body weight was used as a covariate, the LOD scores for femur bone area, periosteal circumference, and endosteal circumference were 6.2, 9.1, and 5.6, respectively. Fine mapping using 8 additional chromosome 4 markers shifted peak linkage for periosteal circumference to 50 cM. It is unclear whether chromosome 4 harbors a single QTL or 2 separate QTL. This locus explains 38%-42% of the phenotypic variance. C57BL/6J-derived alleles at Bnszq1 confer decreased bone size in males. Potential candidate genes are Calb1 (10.5 cM), Igfbpl1, Casp8ap2 (11.4 cM), Gabrr1 (10.5 cM), Gabrr2 (10.5 cM), Cga (10.5 cM), Rragd (11.4 cM), Il11ra1 (12.7 cM), andMusk (26.3 cM).
  
  A second locus named Bnszq2 (bone size QTL 2) mapped to 45 cM (LOD=4.9) on mouse Chromosome 1 and appears to interact with Bnsz1 on chromosome 4. C3H-derived alleles at Bnszq2 confer decreased bone size in males. The combined effect of Bnsz1 and Bnsz2 explains 60% of the phenotypic variance. Previously identified bone QTL mapping near Bnsz2 include Bmd1 (87.9 cM), Bmd5 (81.6 cM), and Pcir1 (84 cM).
  
  A suggestive bone size locus mapped to 5 cM on mouse Chromosome 14 (LOD=4.7) when body weight was used as a covariate.