Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  18
• Reference
  J:70565 Lee GH, et al., Fine chromosomal localization of the mouse Par2 gene that confers resistance against urethane-induction of pulmonary adenomas. Oncogene. 2001 Jul 5;20(30):3979-85
• ID
  MGI:3586648

Genes

Gene	Assay Type
Par2	resistance/susceptibility
D18Mit103	PCR
D18Mit188	PCR
D18Mit141	PCR
D16Mit186	PCR
Dcc	reported elsewhere

Notes

• Experiment
  The Par2 locus was refined to a 0.5 cM interval on mouse Chromosome 18 between D18Mit103 (44 cM) and D18Mit188 (47 cM) by recombinant congenic analysis. Semi-congenic lines were constructed by introgression of the Par2 locus (approximately 25 cM from D18Mit123 to D18Mit144) from adenoma resistant inbred strain BALB/cByJJcl onto the genetic background of adenoma susceptible inbred strain A/JSlc for 4-5 backcross generations. Nine male mice congenic (N6-N7) for segments of the Par2 interval from C57BL/6JJcl on a BALB/cByJJcl background were bred to female A/JSlc mice [(A/JSlc x CByJJcl.B6-Par2^C57BL/6JJcl)F1] and the resulting 197 progeny were used to fine map the locus. Four microsatellite markers showed tight linkage to Par2. D18Mit103 and D18Mit188 flank the Par2 interval while D18Mit141 and D18Mit186 are internal to the Par2 interval.
  
  The BALB/cJJcl-derived allele at Par2 appears to have a dominant effect on urethane-induced lung tumor resistance. The presence of one BALB/cJJcl-derived Par2 allele reduces tumor multiplicity by 65% in male animals and by 51% in female animals.
  
  Dcc at 45 cM maps within the 0.5 cM Par2 interval and is considered a possible candidate gene.