Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  6
• Reference
  J:92838 Ho M, et al., Disruption of muscle membrane and phenotype divergence in two novel mouse models of dysferlin deficiency. Hum Mol Genet. 2004 Sep 15;13(18):1999-2010
• ID
  MGI:3581689

Genes

Gene
Dysf
D6Mit188
D6Mit8
D6Mit284

Notes

• Experiment
  The progressive muscular dystrophy phenotype in A/J animals bred after 1975 was mapped using 52 animals from an A/J x (A/J x C57BL/6J)F1 backcross. These animals were genotyped at 80 polymorphic loci and linkage to the progressive muscular dystrophy phenotype was obesrved near D6Mit8 (32.5 cM) on mouse Chromosome 6. Dysf maps to this locus and was investigated as a candidate gene. Sequence analysis of Dysf revealed a retrotransposon insertion within intron 4 resulting in a null allele specifically in theA/J strain.
  
  Complementation analysis was performed by crossing A/J to SJL-Dysf^im. Resulting F1 animals all exhibit muscular degeneration. This is evidence that the mutation in A/J and the Dysf^im mutation in SJL are allelic.
  
  The order and distance between markers on mouse Chromosome 6 as determined from the A/J x (A/J x C57BL/6)F1 backcross is as follows:
  
  centromere - D6Mit188 - 1.75 +/- 1.74 cM - D6Mit8/Dysf - 5.26 +/- 2.96 cM - D6Mit284 - telomere