Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  6
• Reference
  J:94512 Rosen CJ, et al., Congenic mice with low serum IGF-I have increased body fat, reduced bone mineral density, and an altered osteoblast differentiation program. Bone. 2004 Nov;35(5):1046-58
• ID
  MGI:3526391

Genes

Gene	Assay Type
Igf1sl1	visible phenotype
D6Mit93	PCR amplified length variant
D6Mit150	PCR amplified length variant
Pparg	reported elsewhere
Cav3	reported elsewhere

Notes

• Experiment
  A previously identified QTL named Igf1sl1 was confirmed and fine-mapped in the present study using congenic strain analysis. Igf1sl1 is associated with serum IGF-1 levels and bone mineral density (BMD), and maps to 51 cM on mouse Chromosome 6 in linkage to D6Mit150.
  
  The congenic strain that was created carries a C3H/HeJ-derived interval that contains Igf1sl1 on a C57BL/6J genetic background. This congenic strain is called B6.C3-(D6Mit93-D6Mit150) and the C3H/HeJ-derived region spans approximately 26.3 cM to 51 cM. Although donor strain C3H/HeJ exhibits increased serum IGF-1 levels (25%-30% higher) compared to background strain C57BL/6J, the Igf1sl1 locus confers decreased serum IGF-1 levels when the alleles are from C3H/HeJ. Therefore, congenic strain B6.C3-(D6Mit93-D6Mit150) is expected to have lower serum IGF-1 levels compared to C57BL/6J and that is what is observed (serum IGF-1 is 15%-25% decreased). The congenic also exhibits decreased rate of bone formation and significantly reduced trabecularandcortical BMD of the L5 vertebrae compared to C57BL/6J.
  
  DNA microarray analysis and Q-PCR (quantitative real-time PCR) were used to identify candidate genes within the Igf1sl1 interval. Pparg and Cav3 map within the Ifg1sl1 interval and are differentially expressed between congenic B6.C3-(D6Mit93-D6Mit150) and parental C57BL/6J.