Mapping Data

Experiment

• Experiment
  TEXT
• Chromosome
  3
• Reference
  J:85319 Buchner DA, et al., High-resolution mapping of the sodium channel modifier Scnm1 on mouse chromosome 3 and identification of a 1.3-kb recombination hot spot. Genomics. 2003 Oct;82(4):452-9
• ID
  MGI:3044198

Genes

Gene	Assay Type
Scnm1
D3Umi1	PCR
D3Umi2	PCR
D3Umi3	PCR
D3Umi4	PCR
D3Umi5	PCR
D3Umi6	PCR
D3Umi7	PCR
D3Umi8	PCR
D3Umi9	PCR
D3Umi10	PCR
D3Umi11	PCR
D3Umi12	PCR
D3Umi13	PCR
D3Umi14	PCR
D3Umi15	PCR
D3Umi16	PCR
D3Umi17	PCR
D3Umi18	PCR
D3Umi19	PCR
D3Umi20	PCR
D3Umi21	PCR
D3Umi22	PCR
D3Umi23	PCR
D3Umi24	PCR
D3Umi25	PCR
D3Umi26	PCR
Vps72	reported elsewhere

Notes

• Experiment
  High resolution mapping was performed on Scnm1 using 26 new markers on mouse Chromosome 3 (D3Umi1-D3Umi26). A population of (B6.Cg-Scn8a^medJ x C3H)F2 animals were used for genetic mapping. Homozygosity for the ^medJ allele on a C57BL/6J (susceptible) background results in paralysis and juvenile lethality whereas on a C3H (resistant) background animals display ataxia, chronic tremor, dystonia, muscle weakness, and reduced motor nerve conduction velocity but live a normal lifespan.
  
  Scnm1 mapped to a 950 kb interval on mouse Chromosome 3 between D3Umi5 and D3Umi21. A recombination hotspot was identified distal to Scnm1 between D3Umi20 and D3Umi21 (approximately 1.3 kb in length).
  
  F2 crosses were also carried out between B6.Cg-Scn8a^medJ and 129X1, andbetween B6.Cg-Scn8a^medJ and 129S6. Inbred strains 129X1 and 129S6 both carry the resistant allele of Scnm1.
  
  Four resistant strains (C3H, 129S6, A/J, DBA/2) share a common haplotype across the 950 kb Scnm1 interval compared to susceptible strain C57BL/6J. Resistant strain 129X1 shares a haplotype with C57BL/6J across 4 markers spanning a 150 kb interval of the Scnm1 region. Tcf1 is a candidate gene mapping to the 950 kb Scnm1 interval and SNP analysis using D3Umi9 (located at Val247Ala within Tcf1) wascarried out. Results show that resistant strains A/J, DBA/2, and 129S6 share the same allele as C3H, but resistant strain 129X1 shares the same allele as susceptible strain C57BL/6J. Authors conclude that Tcf1 may be excluded as a candidate gene for Scnm1.