Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  4
• Reference
  J:63674 Tucker RM, et al., Genetic control of glycoprotein 70 autoantigen production and its influence on immune complex levels and nephritis in murine lupus. J Immunol. 2000 Aug 1;165(3):1665-72
• ID
  MGI:3040464

Genes

Gene	Assay Type
Elsgp1	visible phenotype
D4Mit170	PCR amplified length variant

Notes

• Experiment
  Linkage analysis was performed on 152 female (B6.H2^z x NZB/BlNJ)F1 x NZB/BlNJ backcross animals and 163 female (NZB/BlNJ x C57BL/6J)F2 intercross animals to identify QTLs linked to elevated serum gp70 (which is associated with the development of gp70 immune complexes and nephritis). 90 microsatellite markers were used in the genome scan. Parental strain NZB/BlNJ is susceptible to autoimmune nephritis compared to parental strain C57BL/6J.
  
  In both the backcross and intercross populations two significant loci, Elsgp1 (elevated serum gp70 1) and Elsgp2, mapped to 66.6 cM on mouse Chromosome 4 (LOD=11.2 at D4Mit170) and to 40 cM on mouse Chromosome 13 (LOD=11 at D13Mit98), respectively. Elsgp2 maps near a previously identified QTL on chromosome 13 named Yaa1 (35 cM) that is also associated with serum gp70 levels. A third locus, Elsgp3, mapped to 49.6 cM on mouse Chromosome 2 (LOD=4.9 at D2Mit14). NZB/BlNJ-derived alleles confer elevated serum gp70 at all 3 loci with additive inheritance. Animals homozygousfor NZB/BlNJ-derived alleles at both Elsgp1 and Elsgp2 exhibit the highest levels of serum gp70. Elsgp1, Elsgp2, and Elsgp3 together account for 83% of the phenotypic variance in F2 mice. These loci, however, do not show linkage to gp70 immune complex levels.
  
  In the backcross population a previously identified QTL on distal mouse Chromosome 1 named Nba2 was detected in linkage to autoantibody production and nephritis, but not to serum gp70 levels. Nba2 did not show linkage in the F2 population.