Experiment
Previously identified QTLs Pas1 and Pas2 were confirmed and fine mapped using (A/J x C57BL/6) advanced intercross lines (AIL) at the F11 generation. Parental strain A/J is susceptible to lung tumors where as parental strain C57BL/6 is resistant.
Pas1 was resolved to a 1 cM - 1.3 cM interval on mouse Chromosome 6 near Kras with a LOD score > 150 at D6Mit57 (71.7 cM).
1.7.2016 - Curator Note: Because Pas1 was orginally mapped in J:17778, in 1997 using an (C57BL/6J x A/J)F2 intercross, which differs in direction from the cross used here, we consider the current study a separate mapping experiment and have named this QTL Pas15 .
Candidate genes in the region of Pas15 include Lrmp, 4933403M22Rik, 4930469P12Rik, Bcat1 (Eca39), 5133400D11Rik (mHoj-1), and Sspn (Krag). SNP analysis of these candidate genes revealed coding region polymorphisms in Lrmp, 4933403M22Rik, and 4930469P12Rik, but not in Bcat1 (Eca39), 5133400D11Rik (mHoj-1), or Sspn (Krag). Candidate gene 4933403M22Rik is highly associated with Pas15 and is referred to by the authors as Pas1c1 (Pas1 candidate gene 1). Semi-quantitative RT-PCR analysis of 4933403M22Rik revealed the presence of different isoform transcripts in C57BL/6 and A/J.
Pas2 was localized to a 7.6 Mb region on mouse Chromosome 17 between D17Mit23 and D17Mit231 with the strongest association (LOD=3.0) at D17Mit16 (17.4 cM). There may be a possible interaction between Pas1 and Pas2.
1.4.2016 - Curator Note: Because Pas2 was originally mapped in J:21801 using (C57BL/6JOlaHsd x A/JOlaHsd)F2 intercross and backcross populations, which differ in direction from the cross used here, we consider the current study a separate mapping experiment and have named the QTL, Pas19.
Pas3 was not detected in this study using advanced intercross lines.
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