Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  17
• Reference
  J:78423 Haston CK, et al., Bleomycin hydrolase and a genetic locus within the MHC affect risk for pulmonary fibrosis in mice. Hum Mol Genet. 2002 Aug 1;11(16):1855-63
• ID
  MGI:2668889

Genes

Gene	Assay Type
Blmpf1	visible phenotype
D17Mit143	PCR amplified length variant
D17Mit213	PCR amplified length variant
D17Mit198	PCR amplified length variant
D17Mit16	PCR amplified length variant
D17Mit21	PCR amplified length variant
D17Mit13	PCR amplified length variant
D17Mit64	PCR amplified length variant
D17Mit35	PCR amplified length variant
D17Mit50	PCR amplified length variant
D17Mit10	PCR amplified length variant
Tnf	reported elsewhere

Notes

• Experiment
  Genome scan using 190 polymorphic markers at an average spacing of 10 cM was performed on a population of (C57BL/6J x C3Hf/Kam)F2 intercross animals to identify QTLs associated with susceptibility to bleomycin-induced pulmonary fibrosis. Parental strain C57BL/6J is susceptible to bleomycin- induced pulmonary fibrosis whereas parental strain C3Hf/Kam is resistant.
  
  A major locus named Blmpf1 mapped to 17.4 cM (in the H2 region) on mouse Chromosome 17 with peak linkage at D17Mit16 (LOD=17.4). The QTL interval of Blmpf1 was narrowed to a 2.7 cM region between D17Mit175 (17.7 cM) and D17Mit148 (20.4 cM) using a congenic strain designated C3H.SW-H2b.C3H-D17Mit175-D17Mit47. This locus exhibits additive inheritance with C57BL/6J-derived alleles conferring increased incidence of bleomycin-induced pulmonary fibrosis. Blmpf1 explains about 20% of the phenotypic variance. Tnf maps near Blmpf1 and may be a possible candidate gene.
  
  Blmpf2 is a male-specific locus and maps to 28 cM on mouse Chromosome 11 with peak linkage at D11Mit272 (LOD=5.6). The QTL range of Blmpf2 spans 24 cM - 37 cM and is flanked by D11Mit310 and D11Mit5. The C3Hf/Kam-derived allele confers decreased lung fibrosis severity in male animals at Blmpf2. The locus also interacts with Blmpf1 and exhibits additive inheritance. Male animals homozygous for C57BL/6J-derived alleles at both Blmpf1 and Blmpf2 show increased incidence of bleomycin- induced pulmonary fibrosis compared to doubly heterozygous animals. Blmpf2 explains about 8.9% of the phenotypic variance. The activity of Blmh shows a correlation to the phenotype and is considered a viable candidate gene.
  
  A suggestive QTL was detected on mouse Chromosome 8 with a LOD score of 2.78 at D8Mit242 and D8Mit271.