Mapping Data

Experiment

• Experiment
  TEXT-Congenic
• Chromosome
  10
• Reference
  J:82715 Lipoldova M, et al., Susceptibility to Leishmania major infection in mice: multiple loci and heterogeneity of immunopathological phenotypes. Genes Immun. 2000 Feb;1(3):200-6
• ID
  MGI:2656517

Genes

Gene	Assay Type
Lmr5	resistance/susceptibility
Lmr5a	resistance/susceptibility
Lmr5b	resistance/susceptibility
Lmr5c	resistance/susceptibility
D10Mit46
D10Mit14
Lmr5d	resistance/susceptibility
Lmr5e	resistance/susceptibility
Lmr5f	resistance/susceptibility
D10Mit25

Notes

• Experiment
  This study reports on the dissection of genetic and functional aspects of susceptibility to Leishmania major infecton using two contrasting inbred strains BALB/cHeA (susceptible) and STS/A (resistant) and a resistant Recombinant Congenic (RC) Strain, CcS-5/Dem, which carries a random set of 12.5% of genes from the strain STS and 87.5% genes from the susceptible strain BALB/c. The CcS5/Dem strain was highly resistant despite its overall genetic similarity to BALB/c. The genetic dissection of resistance of the RC strain CcS5 was presented in this study.
  
  The F2 hybrids between (BALB/c CcS5) were genotyped with microsatellite markers covering the 12 STS derived segments on eight chromosomes. Linkage analysis of different parameters of the disease revealed five novel loci, Lmr3 - Lmr7, affecting the response to the infection, each associated with a different combination of pathological symptoms and immunological reactions.
  
  02.25.2016 Curator Note: We have retained the broader QTL identified in this study as Lmr5. We have also assigned official nomenclature to each of the independent traits that map with significance within the broader Lmr5 locus creating Lmr5a, Lmr5b, Lmr5c, Lmr5d, Lmr5e and Lmr5f.
  
  Lmr5, leishmaniasis resistance 5, mapped to Chr 10 linked with markers D10Mit46, D10Mit14 and D10Mit25.
  Within this QTL, Lmr5a was associated with larger lesions mapping with marker D10Mit46, corrected p=0.00058.
  Lmr5b, linked with D10Mit25 was associated with higher serum levels of IgE, corrected p=0.0000016.
  Lmr5c, also linked with D10Mit46 was associated IL-12 serum levels, corrected p=0.0085.
  Lmr5d, linked with marker D10Mit25 was associated with higher IFNgamma levels, corrected p=0.018.
  Lmr5e, linked with marker D10Mit46 was associated with splenomegaly, corrected p=0.065.
  The BALB/c allele was associated with larger lesions, higher serum leveles and likely also splenomegaly. [Table 2].
  Lmr5f (D10Mit25) was identified in interactions with Lmr4b (D6Mit10), corrected p=0.011; and with Lmr4c (D6Mit122), corrected p=0.064.
  Homozygous BALB/c alleles at these loci were associated with increases in INFgamma levels. [Table 3].
  
  Ifng, interferon gamma and Stat6, signal transducer and activator of transcription 6 are suggested candidate genes for Lmr5. [Table 5].