Mapping Data

Experiment

• Experiment
  TEXT-Physical Mapping
• Chromosome
  3
• Reference
  J:80336 Di Palma F, et al., Mutations in Mcoln3 associated with deafness and pigmentation defects in varitint-waddler (Va) mice. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14994-9
• ID
  MGI:2652659

Genes

Gene	Assay Type
D3Mit185	overlapping clones, direct sequencing, sequence analysis
D3Mit260	overlapping clones, direct sequencing, sequence analysis
Mcoln3	overlapping clones, direct sequencing, sequence analysis
Mcoln2	overlapping clones, direct sequencing, sequence analysis
Lpar3	overlapping clones, direct sequencing, sequence analysis
D3Mit292	overlapping clones, direct sequencing, sequence analysis
D3Mit259	overlapping clones, direct sequencing, sequence analysis

Notes

• Experiment
  The authors constructed a physical map of the critical Va^J region spanning 350kb. Two overlapping BAC clones RP23-121J1 and RP23-108E10 were sequenced. Sequence analysis revealed the order of markers in the region as: D3Mit85 - D3Mit260 - 5'- Mcoln3 - 3'- 5'- Mcoln2-3' - 5'- Edg7 - 3' - D3Mit292 - D3Mit259 - telomere. Further analysis showed that mutations in Mcoln3 are responsible for the Va^J phenotype.