Mapping Data

Experiment

• Experiment
  TEXT-QTL
• Chromosome
  9
• Reference
  J:72264 Wittenburg H, et al., Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice. Physiol Genomics. 2002 Feb 11;8(1):67-77
• ID
  MGI:2176823

Genes

Gene	Assay Type
Lith5	resistance/susceptibility
D9Mit307
Lipc	reported elsewhere
Scap	reported elsewhere

Notes

• Experiment
  224 (SWR/J x AKR/J)F1 x AKR/J backcross animals were screened for 92 microsatellite markers at an average spacing of 20 cM to detect QTLS associated with gallstone formation. Parental strain SWR/J is susceptible to gallstone formation when fed a lithogenic diet whereas AKR/J is resistant. A significant gallstone QTL, Lith5, was identified on mouse Chromosome 9 in linkage to D9Mit307 at approximately 43 cM. SWR/J-derived alleles confer increased gallstone score in a dominant or partially dominant manner at Lith5. Two candidate genes mapping to the Lith5 locus are Scap (Sreb cleavage-activating protein) and Lipc (hepatic lipase). Sequence analysis of Scap did not reveal differences between SWR/J and AKR/J strains. Lith5 appears to interact with loci at D6Mit14 and D15Mit26 to affect gallstone score. Suggestive loci for gallstone formation were also detected on mouse Chromosomes 1, 2, 6, and 8. A suggestive interacting marker pair, D5Mit100 and D11Mit74, was detected in linkage to gallbladder mucin gel accumulation. All suggestive loci await confirmation and futher investigation.